10.1038/nm.4224įrancis ME, McNeil M, Dawe NJ, Foley MK, King ML, Ross TM, et al. Molecular-level analysis of the serum antibody repertoire in young adults before and after seasonal influenza vaccination. Lee J, Boutz DR, Chromikova V, Joyce MG, Vollmers C, Leung K, et al. Global mortality associated with seasonal influenza epidemics: New burden estimates and predictors from the GLaMOR Project. Paget J, Spreeuwenberg P, Charu V, Taylor RJ, Iuliano AD, Bresee J, et al. (C) Sequence analysis of heavy and light chain private and public mAbs. For each mAb, the minimal concentration needed to inhibit hemagglutination is reported in the corresponding tables and expressed in microgram per milliliter. All mAbs were tested across a 2-fold serial dilution series, starting from 10 μg/mL. (B) HAI activity of mAbs against the corresponding H1 (CA/09), H3 (HK/14) and IBV (Brisb/08) vaccine strains. The area under the curve (AUC) was calculated from each dilution curve and reported on the heatmap table. All the mAbs were tested at different 3-fold dilutions, starting from 20 μg/mL. For H1-, H3- and IBV-specific mAbs the HA1 and HA monomer from CA/09, WI/05 and MA/12 were used, respectively. Binding activity was also tested against COBRA H1N1 P1, X3 and X6 and H3N2 T10 and T11 rHA, the chimeric cH6/1, cH5/3 and cH7/3 rHAs, a truncated HA1 rHA and an rHA monomer. Binding of mAbs was evaluated against a panel of H1, H3 and IBV rHAs from historical seasonal, pandemic (CA/09) and post-pandemic (Mich/15 and Brisb/18) strains. (A) Synopsis of H1-, H3-, and IBV-specific human mAbs binding against H1N1, H3N2 and IBV rHA. Collectively, this high-resolution antibody repertoire analysis demonstrated the impact evolution can have on BCRs in response to influenza virus vaccination, which can guide future universal influenza prophylactic approaches. Binding profiles of one private and three public PBs confirmed they were all subtype-specific, cross-reactive hemagglutinin (HA) head-directed antibodies. Approximately 60% (10/17) of participants experienced convergent evolution, possessing public PBs that were elicited independently in multiple participants. In some participants, a single expanded clonotype accounted for ~22% of their PB BCR repertoire. A combination of Immune Repertoire Capture (IRCTM) technology and IgG sequencing was performed on ~7,800 plasmablast (PB) cells and preferential IgG heavy-light chain pairings were investigated. In this study, PBMCs were collected from 17 human participants vaccinated with the split-inactivated influenza virus vaccine during the 2016-2017 influenza season. Cell Proliferation published by Beijing Institute for Stem Cell and Regenerative Medicine and John Wiley & Sons Ltd.Recent advances in high-throughput single cell sequencing have opened up new avenues into the investigation of B cell receptor (BCR) repertoires. Immune repertoire analysis also allows us to observe participant's wellness, aiding in early-stage diagnosis. Taken together, our study gives us a better understanding of the immune repertoire of different normal Chinese people, and these results can be applied to the treatment of age-related diseases. We further analyzed the degree of complementarity determining region 3 sequence sharing between the two groups, and found shared TCR-alpha, TCR-gamma, immunoglobulin-kappa and immunoglobulin-lambda chain complementarity determining region 3 sequences in all subjects. In addition, we found that the T-cell receptor repertoire was more significantly affected by age than the B-cell receptor repertoire, including significant differences in the use of the unique TCR-alpha and TCR-beta V-J gene combinations, in the two groups of normal participants. We found that compared with the younger group, the clonal expression of T-cell receptor repertoire increased in the older group, while diversity decreased. Normal in this study is defined as no chronic, infectious or autoimmune disease within 6 months prior to blood draw. In this study, all seven receptor chains from both B and T cells in peripheral blood of 16 normal Chinese individuals from two age groups were analyzed using high-throughput sequencing and dimer-avoided multiplex PCR amplification. This study investigated the characteristics of the immune repertoire in normal Chinese individuals of different ages.
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